Immobilization of β-Galactosidase in Adsorptive Membranes for the Continuous Production of Galacto-Oligosaccharides from Lactose§
Larisa Engel1, Philipp Schneider1, Mehrdad Ebrahimi1, Peter Czermak*, 1, 2
Identifiers and Pagination:Year: 2007
First Page: 17
Last Page: 23
Publisher Id: TOFSJ-1-17
Article History:Received Date: 23/7/2007
Revision Received Date: 30/8/2007
Acceptance Date: 10/9/2007
Electronic publication date: 11/10/2007
Collection year: 2007
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A method for immobilization of β-galactosidase from Kluyveromyces lactis on an adsorptive membrane for the continuous synthesis of galacto-oligosaccharides from lactose was carried out. The immobilization was performed at 4, 10, 15 and 40°C. Two strongly basic anion exchange membranes with tradename MustangTMQ and SartobindTMQ were investigated. In static experiments, the highest enzyme activity was measured on MustangTMQ membranes at the immobilization temperature of 10°C. The synthesis of GOS was performed in a Continuous Membrane-Chromatography- Reactor-System (CMCRS) at 40°C and pH 7.0 using 20 wt% initial lactose concentration. The investigated membranes proved to be a good support for the continuous process at high convective flow rates in the enzyme reactor system. Up to 82% lactose conversion with 24% GOS yield was achieved at different fluxes. The corresponding reactor productivity for the production of GOS from lactose in the CMCRS was 98.7 grams GOS per hour and cubic centimeter membrane volume, which significantly exceeds previously reported results.