RESEARCH ARTICLE


Liquid Chromatographic Determination of Malvidin-3-O-Glucoside and Malvidin 3, 5-O-Diglucoside in Wine Samples by Direct Injection



A. Rodríguez-Bernaldo de Quirós, J. López-Hernández*, M.A. Lage-Yusty
Analytical Chemistry, Nutrition and Bromatology Department, Pharmacy Faculty, Campus Sur s/n, University of Santiago de Compostela, 15782 Santiago de Compostela (La Coruña), Spain.


© 2008 Quiróset al.;

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Analytical Chemistry, Nutrition and Bromatology Department, Pharmacy Faculty, Campus Sur s/n, University of Santiago de Compostela, 15782 Santiago de Compostela (La Coruña), Spain; Tel: +34 981 598450; Fax: +34 981 594912; E-mail: qnlhjul@usc.es


Abstract

A method for the determination of malvidin 3-O-glucoside (Oenin), and malvidin 3, 5-O-diglucoside (Malvin) in wines by on line HPLC coupled with UV, and fluorescence detectors is described. With the proposed method the samples were analysed by direct injection without a previous treatment.

For method validation, satisfactory recoveries (<95%) and suitable repeatabilities (within day: R.S.D. (n=6=) > 3%, and between days; R.S.D. (n=6=) >3%) were achieved.

The method was applied to the analysis of commercially available red wines.

Keywords: Chromatographic analysis, direct injection, malvin, oenin, wine.