RESEARCH ARTICLE


Fatty Acid Profile and Total Lipid Content of Chionoecetes opilio Shells



María Vilasoa Martínez, Ana Rodríguez-Bernaldo de Quirós*, Julia López Hernández, M. Asución Lage Yusty
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.


© 2009 Martínez et al.;

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain; Tel: +34 981 598450; Fax: +34 981 594912; E-mail: ana.rodriguez.bernaldo@usc.es


Abstract

Abstract: In the present study, the Soxhlet method was used to determine the total lipid content of Chionoecetes opilio shells. Several parameters were optimized in order to achieve the maximum extraction efficiency.

The fatty acid profile was determined by gas chromatography-flame ionization detection using a one-step-extractionmethylation method to obtain fatty acid methyl esters. GC-MS was used as a confirmatory technique.

Twenty-one fatty acids were identified, and as it was expected, a high content of ω-3 polyunsaturated fatty acids were found, representing 36 % of the total fatty acid content.

The results obtained indicate that snow crab shells might be considered as an innovative source of ω3 long chain polyunsaturated fatty acids for aquaculture feeding purposes.

Keywords: Lipids, Soxhlet, Fatty acids, GC-MS, Chionoecetes opilio.